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无回流A1/O1/A2/O2+潜流式湿地处理农村生活污水效能研究
论文作者:童鞋论文网  论文来源:www.txlunwenw.com  发布时间:2019/9/11 8:33:34  

摘要:本文针对太湖流域地区农村生活污水高标准排放要求(DB32/1072-2017),采用无回流A1/O1/A2/O2+潜流式湿地处理太湖地区农村生活污水。主要有以下几个部分:①构建一套A1/O1/A2/O2/垂直潜流式人工湿地装置,考察组合工艺在不同HRT下对污水中对CODcr、TP、TN 和 NH4+-N 的去除特性,构建动力学模型,并采用荧光光谱结合FRI法考察最佳HRT下DOM组分去除效率;②通过常规指标和高通量测序手段考察冬季低温条件下投加反硝化聚磷菌剂(B8)对A1/O1/A2/O2处理污染物的强化效果及微生物特性;③考察组合工艺对雌酮(E1)、雌二醇(E2)、乙炔基雌二醇(EE2)和雌激素三醇(E3)四种雌激素的去除效率,并采用高通量测序手段研究雌激素对系统的微生物群落结构和功能的影响。④通过常规指标检测和高通测序手段,追踪评价范工程(A1/O1/A2/O2/潜流式人工湿地/生态沟渠(48 t/d))对污染物的去除效能,并分析示范工程与小试的人工湿地细菌群落特性。主要结论如下:

(1)本试验研究得到的最佳水力停留时间为9.5 h(A1/O1/A2/O2)、10.3d(湿地),CODcr、氨氮、总氮和总磷平均去除率分别为78.64%、97.16%、91.84%、95.5%,满足了太湖流域污水的高标准排放要求。各处理单元出水的荧光积分标准体积表示,A1/O1/A2/O2系统对各类溶解性有机污染物都保持较好的去除率,其中芳香类蛋白质(Ⅰ)达到97%,并利用Grau second-order动力学模型拟合得到不同HRT下A1/O1/A2/O2对污染物去除的动力学方程。

(2)冬季低温条件下,A1/O1/A2/O2出水水质下降。通过投加反硝化聚磷菌群对装置B进行生物强化,装置B经过投菌强化后出水水质稳定,其中氨氮出水稳定在0.9-2.8 mg/L,平均去除率提高了约6.7%,TN出水稳定在10 mg/L,平均去除率提高了约13.21%;TP 出水稳定在0.40mg/L,平均去除率提高了约6.9%。缺氧池和好氧池具有一定比例的脱氮菌群,投加反硝化聚磷菌B8后好氧池污泥中的主要脱氮菌属丰度由10.05%增加到20.38%,主要有不动杆菌属、芽殖杆菌属、假单胞菌属、热单胞杆菌属和黄杆菌属等,其中,具有异养硝化反硝化功能和反硝化聚磷的不动杆菌属和假单胞菌属等菌群明显增多,各菌群之间相互协同作用从而强化了系统冬季低温条件下的脱氮除磷能力。

(3)A1/O1/A2/O2+潜流式人工湿地组合工艺对E1、E2、E3和EE2的去除率分别达为96.04%、92.45%、85.09%、88.38%。在整个处理流程中,一级好氧池对4种EDCs的去除贡献最大,湿地系统对于4种EDCs的去除效率可在生物单元基础上提高约23%。加入雌激素后运行的好氧池微生物物种数量要多于加药前,不动杆菌、从毛单胞菌和假单胞菌等硝化反硝化菌属增加成为优势菌属,对系统中雌激素的去除起到促进作用。

(4)示范工程的监测数据显示,A1/O1/A2/O2/潜流式人工湿地/生态沟渠对生活污水有着较好的去除效果,其对CODcr、氨氮、总氮和总磷的平均去除率可达91.7%、97.5%、94.6%和95.3%,出水达到《太湖地区城镇污水处理厂及重点工业行业主要水污染物排放限值》(DB32/1072-2017)。各处理单元出水的荧光积分标准体积表示,A1/O1/A2/O2系统对各类溶解性有机污染物都保持一定的去除率,其中芳香类蛋白质(Ⅰ)达到90%,其它种类的DOM处理效果一般。

(5)脲酶活性分析表示,小试潜流湿地前端的脲酶活性值高于后端;高通量测序结果表明,小试湿地进出水处、示范工程湿地进出水处和生态沟渠中有着丰度的脱氮菌群,相对丰度分别为20.50%、14.16%、8.32%、15.33%和24.51%,其中,具有异养硝化反硝化功能和厌氧反硝化功能的菌属占比较大,如不动杆菌、假单胞菌、芽孢杆菌和黄杆菌等。

Aimed high standards of rural sewagedischarge requirements (DB32/1072-2017) in Taihu Lake Basin area, the no-reflowA1/O1/A2/O2+subsurface wetland is used to treat rural domestic sewage in TaihuLake region.There are mainly the following parts: ①The installation ofA1/O1/A2/O2/subsurface flow wetland was constructed.Then I investigated theremoval characteristics of CODcr, TP, TN and NH4+-N at different HRT, andconstructed a kinetic model to evaluate it. Fluorescence spectroscopy combinedwith FRI method is used to investigate the degradation efficiency of DOMcomponents under optimal HRT. ②Under low temperature in winter, I investigatedthe enhancement effect of contamiant removal and microbial characteristicsafter adding denitrifying phosphorus-concentrating agent (B8) into theA1/O1/A2/O2 device by conventional indicators and high-throughput sequencingmethod. ③The removal efficiency of EDCs (E1,E2,E3,EE2) is investigated ,and themicrobial community structure and function of the system are studied byhigh-throughput sequencing. ④According to the detection of indicators andhigh-throughput sequencing method, I tracked and evaluated the pollutantremoval efficiency of the project (A1/O1/A2/O2/subsurface flow constructedwetland/ecological ditch (48 t/d)),then analyzed the microbial communitycharacteristics of constructed wetlands between site and the laboratorytest.The main conclusions are below:

(1) The optimal HRT obtained in this studyis 9.5 h (A1/O1/A2/O2) and 10.3 d (wetland). The average removal rates ofCODcr, NH4+-N, TN and TP are respectively 78.64%, 97.16% and 91.84%, 90.55%,meeting the high standard discharge requirements of the Taihu Basin.Thestandard volume of fluorescence integral of each treatment unit indicates thatthe A1/O1/A2/O2 system maintains a good removal rate for various dissolved organicpollutants, and the removal rate of aromatic protein (I) reaches 97%.The Grausecond-order dynamics model is used to fit the kinetic equation of A1/O1/A2/O2for pollutant removal under different HRT.

(2) Under the low temperature in winter,the water quality index of A1/O1/A2/O2 effluent decreases. The effluent waterquality is stable after adding denitrifying phosphorus-accumulating bacteria,where the effluent of NH4+-N is stable at 0.9-2.8 mg/L,and the average removalrate increases by about 6.7%;effluent of TN is about 10 mg/L, and the averageremoval rate increases by about 13.21%;effluent of TP is about 0.40 mg/L,andthe average removal rate increases by about 6.9%. The microbial community ofA1/O1/A2/O2 system is analyzed by high-throughput sequencing technology. Theanoxic and aerobic ponds have a certain proportion of denitrifying bacteria.The abundance of the main denitrifying bacteria in the aerobic pond sludgeincreases from 10.05% to 20.38% after adding the denitrifying phosphorus accumulatingbacteria B8, including Acinetobacter, Bacillus, Pseudomonas, Thermomonas, andFlavobacterium,etc. The synergy between the various bacterial groups enhancesthe removal capacity of nitrogen and phosphorus of the system under lowtemperature conditions in winter. 

(3) The removal rates of four estrogens inE1, E2, E3 and EE2 by A1/O1/A2/O2+subsurface flow constructed wetland are96.04%, 92.45%, 85.09% and 88.38%, respectively. The amount of microbialspecies in the aerobic pool after the addition of estrogen is more than thatbefore dosing. Nitrifying-denitrifying bacteria such as acinetobacter,pseudomonas increase to become dominant bacteria, possibly may promote theremoval of estrogen in the system.

(4) The process of A1/O1/A2/O2+subsurfaceflow wetland+ecological ditches is used to treat the village sewage, and theoverall operation is good. The average removal rate of CODcr, NH4+-N, TN and TPcan reach 91.7%, 97.5%, 94.6% and 95.3%, and the effluent reaches Limits ofMajor Water Pollutants in Urban Wastewater Treatment Plants and Key IndustrialSectors in Taihu Lake Area (DB32/1072-2017).

关键词:农村生活污水;无回流A1/O1/A2/O2;潜流式人工湿地;菌剂强化;高通量测序

rural domestic sewage; non-refluxA1/O1/A2/O2; subsurface flow constructed wetland; microbial intensification;high-throughput sequencing

上一篇:狐尾藻腐解释放胡敏酸的特性及对沉水植物生长的影响     下一篇:典型PPCPs在氯氧化及紫外/氯工艺中的降解与转化规律研究
 
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